Coding
Part:BBa_K4601008:Design
Designed by: Doriane Blaise Group: iGEM23_Evry-Paris-Saclay (2023-09-23)
NpHR-NpHtrII-EnvZ
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 1002
Design Notes
Source
DNA synthesis + PCR amplifications from N. pharaonis and E. coli genomes, followed by Golden Gate assembly